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Deuterons Cannot Replace Protons in Active Transport Processes in Yeast

Author: Arnost Kotyk, Milada Dvorakova and Jiri Koryta
Introduction:
A large amount of data on the involvement of protons in various solute transports in yeast has accumulated over the past 15 years even if the evidence was often unconvincing and unsatisfactory in quantitative terms. It could even be argued that the phenomena observed on adding a presumably H+-symported solute to a yeast suspension, such as transient alkalization of the outside medium or depolarization of the membrane, could be explained by assuming the hydrolysis of ATP to be the source of energy for both the proton-extruding ATPase and for the solute transports.

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